Assoc. Prof. RNDr. Vladan Ondřej, Ph.D.

vladan.ondrej@upol.cz

Education and Professional experience:

• 1994-1999 Palacký University, Faculty of Science, Olomouc, biology and chemistry
• 1999-2002 Palacký University, Faculty of Science, Olomouc, PhD study in botany, PhD thesis: Embryo cultures of cultivated and wild species of genus Cucumis (supervisor Prof. A. Lebeda)
• 2002-2003 researcher, Agritec, Ltd.
• 2003-2007 researcher, Institute of Biophysics AS CR
• 2008 researcher, Department of Botany, Palacký University, Faculty of Science, OlomoucOlomouc

Fellowships:

• 2000 Department of Plant Cytology and Embryology, Jagiellonian University in Cracow, Poland
• 2004-2005 Swammerdam Institute for Life Sciences, University of Amsterdam, prof. R. van Driel group

Other professional experience:

• 2002 GMO course, Institute of Plant Molecular Biology AS CR
• 2007 course and license for supervision and disposal of sources of ionizing radiation, State Office for Nuclear Safety

Research activities:

• Protoplast culture, embryogenesis of plants
• Structure and organization of genome, nuclear architecture, DNA repair
• Fluorescence and confocal microscopy, image data analyses

Projects:

• Variability of components and interactions in plant pathosystem and impact of environmental factors on their expression (spolupracovník)
• Modification of histone in chromatin regions with increased and decreased gene expression (spoluřešitel)
• Embryo cultures of wild and cultivated species of genus Cucumis (řešitel)
• 3D Genome (EU project) (spolupracovník)
• Structure of human genome (spolupracovník)
• The use of combination of laser microbeam and cytometric techniques in the study of the structure and dynamics of human genome (spolupracovník)

Publications

• Ondřej, V., Navrátilová, B., Protivánková, I., Piterková, J., Sedlářová, M., Luhová, L. et Lebeda, A. (2010): Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress. Journal of Experimental Botany, 61, 2395-2401.
• Skálová, D., Navrátilová, B., Ondřej, V. et Lebeda, A. (2010): Optimizing culture for in vitro pollination and fertilization in Cucumis sativus and C. melo. Acta Biologica Cracoviensia, Series Botanica, 52, 111-115.
• Lebeda, A., Švábová, L., Ondřej, V., Horáček, J., Kitner, M. et Klenotičová, H. (2010): Fusarium solani and Phoma pisi culture filtrates and their utilization in the selection of resistant pea somaclones; poster P1.262. In: Programme Book, IMC9, The Biology of Fungi, Edinburgh, UK, 1.-6.8.2010; IMC9 Poster Programme (Delegate CD-Rom; www.imc9.info). Published by British Mycological Society, Edinburgh, UK.
• Ondřej, V., Navrátilová, B. et Lebeda, A. (2009): Heterochromatin as a marker for protoplast differentiation of Cucumis sativus. - Plant Cell. Tiss. Org. 96: 229-234.
• Ondřej, V., Kitner, M., Doležalová, I., Nádvorník, P., Navrátilová, B. et Lebeda, A. (2009): Chromatin structural rearrangement during dedifferentiation of protoplasts of Cucumis sativus L. - Mol Cells 27: 443-447.
• Navrátilová, B., Skálová, D., Ondřej, V., Kitner, M. et Lebeda, A. (2009): The novel approaches in the biotechnological research of Cucumis sp. In: The 4th International Cucurbitaceae Symposium, September 20-24, 2009, Changsha, Hunan, China; Abstracts, p. 118. Published by Hunan Agricultural University and International Society for Horticultural Science (ISHS), Changsha, Hunan, China.
• Ondřej, V., Protivánková, I., Doležalová, I. et Lebeda, A. (2009): Structural changes of the chromatin during protoplastization of the plant cells and their regeneration. In: Šperková, R. (Ed.): Analytical Cytometry V. International Conference. Book of Abstracts, September 5-8, 2009, Olomouc, Czech Republic, pp. 53-54. Czech Society for Analytical Cytometry, o.s., Brno. (ISBN 978-80-254-2561-5)
• Havránek, P., Ondřej, V., Kitner, M., Kopecký, P., Wipler, J. et Votruba, P. (2009): “Štěpkový” smrž – co to je? (“Wood chip” morrels – what´s the matter?). – Mykologické Listy, Supplementum, Česko-slovenská vědecká mykologická konference, Brno, 27.-29. srpna 2009, Abstrakty, p. 70-71, Praha. (ISBN 978-80-254-6038-2, ISSN 1213-5887)
• Ondřej, V., Havránek, P. et Kitner, M. (2009): Komparativní analýza sekvenačních dat Českých a světových izolátů smržovitých hub (Comparative analysis of sequences data of Czech and world isolates of the morels). – Mykologické Listy, Supplementum, Česko-slovenská vědecká mykologická konference, Brno, 27.-29. srpna 2009, Abstrakty, p. 49-50, Praha. (ISBN 978-80-254-6038-2, ISSN 1213-5887)
• Ondřej, V., Lukášová, E., Krejčí, J. et Kuzubek, S. (2008): Intranuclear trafficking of plasmid DNA is mediated by nuclear polymeric proteins lamins and actin. - Acta Biochim. Pol. 55: 307-315.
• Ondřej, V., Lukášová, E., Krejčí, J., Matula, P. et Kozubek, S. (2008): Lamin A/C and polymeric actin in genome organization. - Mol. Cells 26: 356-361.
• Ondřej, V., Lukasová, E., Falk, M. et Kozubek, S. (2007): The role of actin and microtubule networks in plasmid DNA intracellular trafficking.- Acta Biochim. Pol. 54: 657-663.
• Falk, M., Lukasová, E., Gabrielová, B., Ondřej, V. et Kuzubek, S. (2007): Chromatin dynamics during DSB repair. BBA Mol. Cell Res. 1773, 1534-1545.
• Goetze, S., Mateos-Langerak, J., Gierman, H.J., de Leeuw, W., Giromus, O., Indemans, M.H.G., Koster, J., Ondřej, V., Versteeg, R. et van Driel, R. (2007): The 3D structure of human interphase chromosome is related to the transcriptome map.- Mol. Cell. Biol., 27: 4475-4487.
• Ondřej, V., Kozubek, S., Lukasová, E., Falk, M., Matula, Pa., Matula, Pe. et Kozubek, M. (2006): Directional motion of foreign plasmid DNA to nuclear HP1 foci.- Chrom. Res. 14: 505-514.
• Lukasová, E., Kořistek, Z., Falk, M., Kozubek, S., Grigoryev, S., Kozubek, M., Ondřej, V. et Kroupová, I. (2005): Methylation of histones in myeloid leukemias as a potential marker of granulocyte abnormalities.- J. Leuk. Biol., 77: 100-111.
• Švábová, L., Smýkal, P., Griga, M. et Ondřej, V. (2005): Agrobacterium-mediated transformation of Pisum sativum in vitro and in vivo.- Biol. Plant. 49: 361-370.
• Vařecha, M., Amrichová, J., Ondřej, V., Lukasova, E., Kozubek, S. et Kozubek, M. (2004): Automated in vivo imaging of cell interior using fluorescent proteins.- Cytometry part A, 59A: 107-107.
• Lanta, V., Havránek, P. et Ondřej, V. (2003): Inter - species hybridization and geneflow in the complex „crop - weed“ during amaranth cultivation.- Plant Soil Environ. 49: 364-369.
• Ondřej, V., Navrátilová, B. et Lebeda, A. (2002): Role of the growth regulators in cucumber zygotic embryogenesis. Abstracts. Sexual Plant Reproduction in Nature and Laboratory, 109.
• Ondřej, V., Navrátilová, B. et Lebeda, A. (2002): In vitro cultivation of Cucumis sativus ovules after fertilization. Proc. 2nd IS on Cucurbits, Nishimura a kol. (eds). Acta Hort. 588: 339-343.
• Ondřej, V., Navrátilová, B., Tarkowski P., Doležal K. et Lebeda, A. (2002): In vitro pollination as a tool of overcoming crossing barriers between Cucumis sativus L. and Cucumis melo L. Acta Fac. Rerum Nat. Univ. Comenianae Bot. 41: 81-88.
• Ondřej, V., Navrátilová, B. et Lebeda, A. (2002): Influence of GA3 on the zygotic embryogenesis of Cucumis species.- Biologia 57: 523-525.
• Lebeda, A., Navrátilová, B. et Ondřej, V. (2001): Effect of GA3 on the embryo cultures of Cucumis species. In: Salaj, J., Obert, B., Salaj, T. (Eds.): From Gametes to Embryos. Xth International Conference on Plant Embryology, September 5-8, 2001, Nitra (Slovak Republic), Book of Abstracts, p. 119.
• Lebeda, A., Navrátilová, B. et Ondřej, V. (2001): In vitro cultivation of Cucumis sativus ovules after fertilization. 2nd International Symposium on Cucurbits, September 28-October 1, 2001, Epochal, Tsukuba (Japan), Abstracts, p. 101.
• Lebeda, A., Navrátilová, B. et Ondřej, V. (2000): Embryo cultures of wild and cultivated species of the genus Cucumis L. Acta Hort. 510, 409-414, 2000.
• Lebeda, A., Navrátilová, B. et Ondřej, V. (2000): Determination of the crossing barriers in hybridization of Cucumis sativus and Cucumis melo. Cucurbit Genetics Cooperative Report 24: 1-5.
• Ondřej, V. et Navrátilová, B. (2000): Influence of genotype and culture medium on embryocultures of Cucumis sativus L. and Cucumis melo L. - Acta Biologica Cracoviensia, 42: 79-81.
• Ondřej, V., Navrátilová, B. et Lebeda, A. (2000): Embryocultures of wild and cultivated species of the genus Cucumis L. Proceedings of Cucurbitaceae 2000. ISHS, Acta Horticulturae, 510: 409-414, 2000
• Ondřej, V., Navrátilová, B. et tarkowski (2000): Obtaining of Cucumis sativus haploid plants via embryo rescue method. Študentská vedecká konferencia 11.12.4. 2000, Bratislava, Sborník abstraktů, 41, 2000
• Ondřej, V. et Navrátilová, B. (1999): Embryocultures of Cucumis sativus L. and Cucumis melo L. PL ISSN, Acta Biologica Cracoviensia, 41: suppl. 1, 56, 1999

Teaching activities

• General biology