RNDr. Ivana Doležalová, Ph.D.

ivana.dolezalova_at_upol.cz

Educational background

• 1985-1990: Palacký University, Faculty of Science, Olomouc, Czech Republic; 1985-1990: Systematical Biology and Ecology; MSc. thesis: Compounds of fluorescence brightening in microscopical diagnostics of mycotic diseases of humans and animals
• 1998-2002: Palacký University, Faculty of Science, Department of Botany, Olomouc, Czech Republic; 1998-2002: PhD study in botany, thesis: Comparative study of selected Lactuca spp. germplasm and their geographical distribution (supervisor: Prof. Ing. A. Lebeda, DrSc.)

Professional Experience

• 1990-1991: assistant in supply division Loana Rožnov p. R., Ltd.
• 1991-1998: maternity leave
• 2002-: senior lecturer, Dept. of Botany, Faculty of Science, Palacký University, Olomouc

Study abroad

• 1999: Austria, Italy, France, Switzerland, Germany; field and collecting expedition
• 2000: Wageningen Agricultural University, Herbarium Vadense, Department of Plant Taxonomy, The Netherlands
• 2002: Canada, USA: IHC, Toronto; Iowa State University, Ames, Iowa; Wisconsin State University, Madison, Wisconsin; USDA and University of California, Department of Vegetable Crops, Davis, CA

Research activities

• Comprehensive study of Lactuca spp. genetic resources (morphological, anatomical, cytological and biochemical aspects)
• Study of geographic distribution, biodiversity and ecobiology of wild Lactuca spp. in natural habitats
• documentation of germplasm of cultivated plants
• Study of variability DNA in germplasm of cultivated plants (flow-cytometry)

Currently running projects

• Variability of components and interactions in plant pathosystem and impact of environmental factors on their expression (spolupracovník)
• EU GENE-MINE (QLK5-2000-00722; Quality of life and management of living resources,
  Key action 5.1.1. Sustainable agriculture, Sustainable management of resources in agriculture) „Improved use of germplasm collections
  with the aid of novel methodologies for integration, analysis and presentation of genetic data sets“ (spoluřešitel)

Publications

• Doležalová, I., Duchoslav, M. et Dušek, K. (2013): Biology and yield of rocket (Eruca sativa Mill.) under field conditions of the Czech Republic (Central Europe). - Not. Bot. Horti. Agrobo. 41: 530-537.
• Beharav, A., Maras, M., Kitner, M., Šuštar-Vozlič, J., Sun, G.L., Doležalová, I., Lebeda, A. et Meglič, V. (2010): Comparison of three genetic similarity coefficients based on dominant markers from predominantly self-pollinating species. Biologia Plantarum 54: 54-60.
• Beharav, A., Ben-David, R., Doležalová, I. et Lebeda, A. (2010): Eco-geographical distribution of Lactuca aculeata natural populations in northeastern Israel. Genetic Resources and Crop Evolution 57, 679-686.
• Kopecký, P., Doležalová, I., Duchoslav, M. et Dušek, K. (2010): Studium odolnosti evropských odrůd kedlubnu vůči nádorovitosti brukvovitých [Study of European kohlrabi varieties for resistance to clubroot disease]. - Úroda 12, vědecká příloha, p. 183-186.
• Beharav, A., Ben-David, R., Malarz, J., Stojakowska, A., Michalska, K., Doležalová, I., Lebeda, A. et Kisiel, W. (2010): Variation of sesquiterpene lactones in Lactuca aculeata natural populations from Israel, Jordan and Turkey. Biochemical Systematics and Ecology, 38, 606-611.
• Lebeda, A., Doležalová, I., Kitner, M. et Novotná, A. (2010): North American continent – a new source of wild Lactuca spp. germplasm variability for future lettuce breeding. In: Rallo, L. et al. (Eds.): Book of Abstracts. Volume II (Symposia). 28th International Horticultural Congress, Lisbon Congress Centre, 22.-27.8.2010, Portugal. ISHS, Lisboa, p. 537.
• Lebeda, A., Křístková, E., Doležalová, I., Petrželová, I. et Melichaříková, Z. (2010): Germplasm collections of old lettuce cultivars and landraces for modern breeding. In: Rallo, L. et al. (Eds.): Book of Abstracts. Volume II (Symposia). 28th International Horticultural Congress, Lisbon Congress Centre, 22.-27.8.2010, Portugal. ISHS, Lisboa, p. 560.
• Lebeda, A., Doležalová, I., Křístková, E., Kitner, M., Petrželová, I., Mieslerová, B., Novotná, A. et Korbelova, P. (2010): Biodiversity of wild Lactuca spp., their evaluation and exploitation. In: Dolenc Koce, J., Vodnik, D., Pongrac, P. (Eds.): Book of Abstracts, 5th Slovenian Symposium on Plant Biology with international participation; 6.-9.9.2010, Ljubljana. Published by Slovenian Society of Plant Biology, Printed by Tiskarna Januš, Ljubljana, pp. 35.
• Křístková, E., Lebeda, A., Doležalová, I., Kitner, M., Petrželová, I., Mieslerová, B., Korbelová, P., Beharav, A., Nevo, E., Ben-David, R., Meglic, V. et Maras, V. (2010): Willow-leaf lettuce – distribution and variation. In: Dolenc Koce, J., Vodnik, D., Pongrac, P. (Eds.): Book of Abstracts, 5th Slovenian Symposium on Plant Biology with international participation; 6.-9.9.2010, Ljubljana. Published by Slovenian Society of Plant Biology, Printed by Tiskarna Januš, Ljubljana, pp. 47.
• Ondřej, V., Kitner, M., Doležalová, I., Nádvorník, P., Navrátilová, B. et Lebeda, A. (2009): Chromatin structural rearrangement during dedifferentiation of protoplasts of Cucumis sativus L. - Mol Cells 27: 443-447.
• Lebeda, A., Doležalová, I., Křístková, E., Kitner, M., Petrželová, I., Mieslerová, B. et Novotná, A. (2009): Comprehensive strategy for searching of valuable traits in wild Lactuca germplasm. In: Meglič, V., Bastar, M.T. (Eds.): Book of Abstracts, 19th EUCARPIA Conference, Genetic Resources Section, May 26-29, Ljubljana, Slovenia. Kmetijski Inštitut Slovenije (Agricultural Institute of Slovenia), Ljubljana, Slovenia, p. 13. (ISBN 978-961-6505-40-6)
• Novotná, A., Doležalová, I., Křístková, E., Berka, T. et Lebeda, A. (2009): Comparative study of achenes characters within genetic resources of Lactuca serriola from Slovenia and Sweden. In: Meglič, V., Bastar, M.T. (Eds.): Book of Abstracts, 19th EUCARPIA Conference, Genetic Resources Section, May 26-29, Ljubljana, Slovenia. Kmetijski Inštitut Slovenije (Agricultural Institute of Slovenia), Ljubljana, Slovenia, p. 45. (ISBN 978-961-6505-40-6)
• Křístková, E., Doležalová, I. et Lebeda, A. (2009): Morphological grouping of Lactuca saligna germplasm originating from Italy and France. In: Meglič, V., Bastar, M.T. (Eds.): Book of Abstracts, 19th EUCARPIA Conference, Genetic Resources Section, May 26-29, 2009, Ljubljana, Slovenia. Kmetijski Inštitut Slovenije (Agricultural Institute of Slovenia), Ljubljana, Slovenia, p. 46. (ISBN 978-961-6505-40-6)
• Doležalová, I., Lebeda, A., Špoková, M. et Křístková, E. (2009): Comparison of phenotypic variability of Lactuca serriola genetic resources from Slovenia and Sweden. In: Meglič, V., Bastar, M.T. (Eds.): Book of Abstracts, 19th EUCARPIA Conference, Genetic Resources Section, May 26-29, 2009, Ljubljana, Slovenia. Kmetijski Inštitut Slovenije (Agricultural Institute of Slovenia), Ljubljana, Slovenia, p. 51. (ISBN 978-961-6505-40-6)
• Michalska, K., Stojakowska, A., Malarz, J., Doležalová, I., Lebeda, A. et Kisiel, W. (2009): Systematic implications of sesquiterpene lactones in Lactuca species. Biochemical Systematics and Ecology, 37, 174-179 (Doi: 10.1016/j.bse.2009.02. 001)
• Lebeda, A., Doležalová, I., Křístková, E., Kitner, M., Petrželová, I., Mieslerová, B. et Novotná, A. (2009): Wild Lactuca germplasm for lettuce breeding: current status, gaps and challenges. Euphytica 170, 15-34. (DOI: 10.1007/s10681-009-9914-7)
• Kitner, M., Maras, M., Lebeda, A., Meglič, V., Šmachová, P., Novotná, A. et Doležalová, I. (2009): Genetic diversity analysis of wild Lactuca serriola germplasm collection from North America assessed by AFLP. In: Goličnik, M., Bavec, A. (Eds.): Joint Congress of The Slovenian Biochemical Society and The Genetic Society of Slovenia with International Participation; Otočec, September 20-23, 2009; Book of Abstracts, p. 175. Published by Slovenian Biochemical Society and Genetic Society of Slovenia, Printed by Ulčakar & JK, Ljubljana (ISBN 978-961-91651-5-7)
• Ondřej, V., Protivánková, I., Doležalová, I. et Lebeda, A. (2009): Structural changes of the chromatin during protoplastization of the plant cells and their regeneration. In: Šperková, R. (Ed.): Analytical Cytometry V. International Conference. Book of Abstracts, September 5-8, 2009, Olomouc, Czech Republic, pp. 53-54. Czech Society for Analytical Cytometry, o.s., Brno. (ISBN 978-80-254-2561-5)
• Doležalová, I., Pavlíček, T., Gasmanová, N., Nevo, E., Hammerová, I. et Lebeda, A. (2009): Microgeographic genome size differentiation of the wild barley, Hordeum spontaneum, at „Evolution Canyon“, Israel and multispecies comparison. In: Šperková, R. (Ed.): Analytical Cytometry V. International Conference. Book of Abstracts, September 5-8, 2009, Olomouc, Czech Republic, pp. 158-159. Czech Society for Analytical Cytometry, o.s., Brno. (ISBN 978-80-254-2561-5)
• Skálová, D., Navrátilová, B., Doležalová, I. et Lebeda, A. (2009): Mixoploid and haploid (pollen) protoplasts of Cucumis spp. 8th International Symposium in the Series Recent Advances in Plant Biotechnology, „New Developments in Green Gene Technology“, 1-4 September 2009, Szeged, Hungary. Program and Abstracts, pp. 75-76. Published by Biological Research Center, Hungarian Academy of Sciences, Szeged, Hungary (http://prof-congress.hu/2009/biotech-8).
• Lebeda, A., Kitner, M., Dziechciarková, M., Doležalová, I., Křístková, E. et Lindhout, P. (2009): An insight into the genetic polymorphism among European populations of Lactuca serriola assessed by AFLP. Biochemical Systematics and Ecology 37, 597-608. (doi:10.1016/j.bse.2009.10.010)
• Lebeda, A., Doležalová, I., Valentová, K., Gasmanova, N., Dziechciarkova, M. et Ulrichova, J. (2008): Yacon (Smallanthus sonchifolius) - A traditional crop of Andean indians as a challenge for the future – The news about biological variation and chemical substances content. Acta Hort., 765, 127-136.
• Doležalová, I., Lebeda, A. et Vondráková, E. (2008): Current status of the Lactuca working collection of Palacký University in Olomouc, Czech Republic. In: Maggioni, L., Lebeda, A., Boukema, I., Lipman, E. (Eds.): Report of a Working Group on Leafy Vegetables. First Meeting, 13-14 October 2005, Olomouc, Czech Republic. Bioversity International, Rome, Italy, 2008, pp. 27-33.
• Treuren van, R., Lebeda, A., Křístková, E., Dehmer, K., Wiel van de, C.C.M., Pink, D., Doležalová, I., Sretenovič Rajicic, T., Astley, D., Spence, N. et van Hintum, Th.J.L. (2008): Overview of studies on wild crop relatives of lettuce carried out within the framework of the EU project GENE-MINE. In: Maggioni, L., Lebeda, A., Boukema, I., Lipman, E. (Eds.): Report of a Working Group on Leafy Vegetables. First Meeting, 13-14 October 2005, Olomouc, Czech Republic. Bioversity International, Rome, Italy, 2008, pp. 41-43.
• Křístková, E., Doležalová, I., Lebeda, A., Vinter, V. et Novotná, A. (2008): Description of morphological characters of lettuce (Lactuca sativa L.) genetic resources. Horticultural Science (Prague), 38, 2008, 113-129.
• Lebeda, A., Doležalová, I., Křístková, E., Kitner, M., Petrželová, I., Mieslerová, B. et Novotná, A. (2008): Wild Lactuca germplasm for lettuce breeding – recent status, gaps and challenges. In: Prohens, J., Badenes, M.L. (Eds.): Modern Variety Breeding for Present and Future Needs. Editorial Universidad Politécnica de Valencia, Valencia, Spain, pp. 49-60.
• Beharav, A., Lebeda, A., Doležalová, I. et Ben-David, R. (2008): Collecting of genetic diversity in natural populations of wild plant species: Case study on Lactuca saligna and L. aculeata in Israel. In: Prohens, J., Badenes, M.L. (Eds.): Modern Variety Breeding for Present and Future Needs. Editorial Universidad Politécnica de Valencia, Valencia, Spain, pp. 75-76. (Abstract).
• Kitner, M., Lebeda, A., Doležalová, I., Maras, M., Křístková, E., Nevo, E., Pavlíček, T., Meglic, V. et Beharav, A. (2008): AFLP analysis of Lactuca saligna germplasm collections from four European and three Middle Eastern countries. - Israel J. Plant Sci. 56: 185-193.
• Beharav, A., Ben-David, R., Doležalová, I. et Lebeda, A. (2008): Eco-geographical distribution of Lactuca saligna natural populations in Israel. Israel Journal of Plant Sciences 56, 195-206.
• Lebeda, A., Ryder, E.J., Grube, R., Doležalová, I. et Křístková, E. (2007): Lettuce (Asteraceae; Lactuca spp.), Chapter 9, pp. 377-472. In: Singh, R. (Ed.): Genetic Resources, Chromosome Engineering, and Crop Improvement Series, Volume 3 – Vegetable Crops. CRC Press, Boca Raton, FL, USA.
• Gasmanová, N., Lebeda, A., Doležalová, I. et Paris, H.S. (2007): Variability of nuclear DNA content among and within three cucurbit genera. Acta Hort. 731, 83-88.
• Beharav, A., Lebeda, A., Petrželová, I., Doležalová, I. et Nevo, E. (2007): Variation in response to Bremia lactucae through natural populations of Lactuca saligna collected in Israel. In: EUCARPIA Leafy Vegetables 2007, Conference Abstracts, 18-20 April 2007, University of Warwick, Warwick HRI, UK, Oral Presentations, p. 9.
• Lebeda, A., Doležalová, I. et Křístková, E. (2007): Identification of duplicates in Lactuca germplasm collections. In: EUCARPIA Leafy Vegetables 2007, Conference Abstracts, 18-20 April 2007, University of Warwick, Warwick HRI, UK; Oral Presentations, p. 21.
• Doležalová, I., Lebeda, A., Novotná, A. et Kršková, M (2007): Variation in fruit morphology of Lactuca serriola populations from Czech Republic, Germany, Netherlands and United Kingdom. In: EUCARPIA Leafy Vegetables 2007, Conference Abstracts, 18-20 April 2007, University of Warwick, Warwick HRI, UK; Poster Presentations, p. 8.
• Kitner, M., Lebeda, A., Doležalová, I., Křístková, E., Beharav, A., Nevo, E., Pavlíček, T., Maras, M. et Meglic, V. (2007): AFLP analysis of Lactuca saligna germplasm collections from four European and three Near East countries. In: EUCARPIA Leafy Vegetables 2007, Conference Abstracts, 18-20 April 2007, University of Warwick, Warwick HRI, UK; Poster Presentations, p. 14.
• Křístková, E., Lebeda, A. et Doležalová, I. (2007): Phenotypic variability of Lactuca saligna germplasm collected in Italy and France. In: EUCARPIA Leafy Vegetables 2007, Conference Abstracts, 18-20 April 2007, University of Warwick, Warwick HRI, UK; Poster Presentations, p. 15.
• Křístková, E., Lebeda, A., Doležalová, I., Vinter, V. et Křístková, A. (2007): Variation in developmental stages of Lactuca serriola (prickly lettuce) germplasm from different European countries. In: EUCARPIA Leafy Vegetables 2007, Conference Abstracts, 18-20 April 2007, University of Warwick, Warwick HRI, UK; Poster Presentations, p. 16.
• Lebeda, A., Doležalová, I., Křístková, E., Dehmer, K.J., Astley, D., van de Wiel, C.C.M. et van Treuren, R. (2007): Acquisition and ecological characterization of Lactuca serriola L. germplasm collected in the Czech Republic, Germany, the Netherlands and United Kingdom. Genet. Res. Crop Evol. 54, 555-562.
• Lebeda, A., Doležalová, I., Křístková, E. et Novotná, A. (2007): Taxonomic determination of plant genetic resources – impact and consequences: case study of Lactuca spp. In: Hauptvogel, P., Benediková, D., Hauptvogel, R. (Eds.): Plant Genetic Resources and their Exploitation in the Plant Breeding for Food and Agriculture. Book of Abstracts. 18th EUCARPIA Genetic Resources Section Meeting, May 23-26, Piešťany, Slovak Republic. PNprint, s.r.o., Piešťany, Slovak Republic, pp. 39-40.
• Křístková, E., Lebeda, A. et Doležalová, I. (2007): Genetic resources of Lactuca saligna and their morphological assessment. In: Hauptvogel, P., Benediková, D., Hauptvogel, R. (Eds.): Plant Genetic Resources and their Exploitation in the Plant Breeding for Food and Agriculture. Book of Abstracts. 18th EUCARPIA Genetic Resources Section Meeting, May 23-26, Piešťany, Slovak Republic. PNprint, s.r.o., Piešťany, Slovak Republic, p. 93.
• Lebeda, A., Doležalová, I., Křístková, E., Mieslerová, B., Kitner, M., Navrátilová, B., Duchoslav, M. et Havránek, P. (2007): Germplasm collections of crop wild relatives – Research, study and use on the Department od Botany, Palacký University in Olomouc (Czech Republic). In: Hauptvogel, P., Benediková, D., Hauptvogel, R. (Eds.): Plant Genetic Resources and their Exploitation in the Plant Breeding for Food and Agriculture. Book of Abstracts. 18th EUCARPIA Genetic Resources Section Meeting, May 23-26, Piešťany, Slovak Republic. PNprint, s.r.o., Piešťany, Slovak Republic, pp. 94-95.
• Doležalová, I., Lebeda, A., Gasmanová, N., Pavlíček, T., Nevo, E. et Hammerová, I. (2007): Intraspecific DNA content variability in wild barley (Hordeum spontaneum (C. Koch) Thell.) in relation to microclimate conditions. In: Analytical Cytometry IV, Book of Abstracts. International Conference, Brno/South Moravia (Czech Republic), June 23-26, 2007. Czech Society for Analytical Cytology, Brno, pp. 98-99. ISBN 978-80239-9591-6.
• Doležalová, I., Lebeda, A., Křístková, E. et Novotná, A. (2007): Relevance of morphologic assessment of wild Lactuca spp. germplasm for their taxonomic determination. Bulletin of Botanical Gardens, Museums & Collections, Polish Botanical Society, vol. 16A, p. 22, 2007. Programme and Abstracts - The 2nd International Conference of Eastern and Central European Botanic Gardens „EastCentGard II“ „European botanic gardens together towards the implementation of plant conservation strategies“, 3-7 July 2007, Warsaw/Rogów, Poland. Botanical Garden – Center for Biological Diversity Conservation of the Polish Academy of Science, Warsaw. (ISSN 1230-3291)
• Gasmanová, N., Lebeda, A., Doležalová, I., Cohen, T., Pavlíček, T., Fahima, T. et Nevo, E. (2007): Genome size variation of Lotus peregrinus at Evolution Canyon I microsite, Lower Nahal Oren, Mt. Carmel, Israel. Acta Biologica Cracoviensia Series Botanica, 49, 39-46.
• Lebeda, A., Mieslerová, B., Sedlářová, M., Rybka, V., Petrželová, I. et Doležalová, I. (2007): Nově potvrzené výskyty padlí na okrasných rostlinách v České republice (New records of powdery mildew on ornamental plants in the Czech Republic). In: Nováková, A. (Ed.): Proceedings of the Workshop MICROMYCO 2007. Institute of Soil Biology, Biological Centre, Academy of Sciencev of the Czech Republic, v.v.i., České Budějovice, pp. 74-80. (ISBN 978-80-86525-10-5)
• Lebeda, A., Doležalová, I., Křístková, E. et Novotná, A. (2007): Comparative study of variation of some morphological characteristics of Lactuca serriola germplasm collected in Central Europe (Czech Republic) and the British Isles (England, U.K.). In: Bullitta, S. (Ed.): Plant Genetic Resources of Geographical and „other“ Islands (conservation, evaluation and use for plant breeding). Proceedings of the XVII Eucarpia Genetic Resources Section Meeting; 30 March – 2 April 2005, Castelsardo (Italy). CNR-ISPAAM sez. Sassari Publisher, Sassari, Italy, pp. 95-96 (Abstract). ISBN: 88-901771-3-6.
• Valentová, K., Lebeda, A., Doležalová, I., Jirovský, D., Simonovska, B., Vovk, I., Kosina, P., Gasmanová, N. et Dziechciarkova, M., Ulrichová J. (2006): The biological and chemical variability of yacon. - J. Agric. Food Chem. 54: 1347-1352.
• Lebeda, A., Doležalová, I., Valentová, K., Gasmanová, N., Dziechciarkova, M. et Ulrichova, J. (2006): Yacon (Smallanthus sonchifolius) - A traditional crop of Andean indians as a challenge for the future – The news about biological variation and chemical substances contents. Abstracts, 27th International Horticultural Congress & Exhibition, August 13-19, 2006, COEX (Convention & Exhibition), Seoul, Korea; ISHS and KSHS, 2006; S03-P-18, p. 85-86.
• Lebeda, A., Doležalová, I., Křístková, E. et Dehmer K.J., Astley D. van de Wiel C.C.M., van Treuren R. (2006): Acquisition and ecological characterization of Lactuca serriola L. germplasm collected in the Czech Republic, Germany, the Netherlands and United Kingdom. Genetic Resources and Crop Evolution, web site ze dne 23.10.2006, http://dx.doi.prg/10..1007/s10722-006-0012-6.
• Lebeda, A., Křístková, E., Doležalová, I., Mieslerová, B., Dančák, M., Duchoslav, M. et Havránek, P. (2006): Ex situ conservation of wild plant species and research programs in Botanic Garden of Palacký University in Olomouc (Czech Republic), In: Hanzelka P. (ed) EuroGard IV, Botanic Gardens and the 2010 Challenge, Congress proceedings, 18th - 22th September 2006, Prague – Průhonice, Czech Republic, Union of Botanic Gardens of the Czech Republic, p. 18.
  
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Teaching activities

• Conservation of genetic resources
• Plant variation and evolution