Asoc. Prof. RNDr. Michaela Sedlářová, Ph.D.

michaela.sedlarova(at)upol.cz

Educational background

• 1995 B.Sc. (Systematic biology and ecology) Palacký University in Olomouc
• 1997 M.Sc. (Botany) Palacký University in Olomouc
  Diploma work (Supervisor: Assoc. Prof. Pavla Binarová, Inst. of Exp. Botany ASCR): Immunocytochemical and cytochemical localisation of pyranose-2-oxidase in cells of wood-decay fungus Phanerochaete chrysosporium Burds. and further electrophoretic characterisation of P2O
• 1998 Education and teaching of biology, Palacký University in Olomouc
• 2001 Ph.D. (Botany) Palacký University in Olomouc
  Thesis (Supervisor: Prof. Aleš Lebeda, Department of Botany, Palacký University): Cytological changes and the role of cytoskeleton in plants during pathogenesis of biotrophic parasites
• 2009 Assoc. Prof. (Phytopathology) Mendel University of Agriculture and Forestry in Brno. Thesis: Bremia lactucae pathogenesis on Lactuca spp.

Professional Experience

• 2000-: Dept. Botany, Dept. Biochemistry, Faculty of Sciences, Palacký University

Membership in scientific societies

• Czechoslovak Biological Society
• Czechoslovak Microscopy Society
• European Microscopy Society
• Czech Society for Plant Pathology
• Czech Scientific Society for Mycology
• Actaea

Research activities

• phytopathogenic oomycetes and fungi
• Lactuca spp.–Bremia lactucae interactions
• plant cytoskeleton during pathogenesis
• phenolic compounds in plant resistance
• aspects of oxidative stress caused by plant pathogens
• growth regulators influence on plant resistance and pathogen development

Study abroad

• 2001: Institute of Botany BAS, Sofia, Bulgaria
• 2002: course „Signals involved in Plant-Microbe Interactions“ (Institute of Biochemistry and Biophysics PAS, Warsaw, Poland)
• 2003: Horticulture Research International Wellesbourne, Warwick, U.K.
• 2004: Dept. Plant Systems Biology, Gent University, Belgium
• 2007: Dept. Plant Protection, Szent István University, Gödölö, Hungary
• 2008: Dept. Scientific and Technological, Università degli Studi, Verona, Italy

Tutor of Bc and MSc studies

• 2011-12: Localization of reactive oxygen and nitrogen species in powdery mildew during its pathogenesis (Klára Křížková)
• 2009-11: Confocal microscopy in RNOS detection (Zuzana Tulpová)
• 2008-10: Pathological physiology of sunflower – interaction with Plasmopara halstedii (Zuzana Trojanová)
• 2007-9: Study on sunflower-Plasmopara halstedii interactions (Karel Stojaspal)
• 2007-9: Peroxidase in the interactions of legumes with fungal pathogens (Gabriela Skalová)
• 2007-8: Modifications of plant morphology and anatomy induced by fungal pathogens (Zuzana Trojanová)
• 2006-7: Variability of Rhododendron powdery mildew teleomorph in the Czech Republic (Karel Stojaspal)
• 2005-8: Host response to pathogen attack: Phenolics and PAL activity (Pavla Mikšíková)
• 2002-2004: Growth regulators influence on the process of biotrophic parasites pathogenesis (Martina Výtisková)

Currently running projects

• Inovation of the metods for sunflower protection (spoluřešitel)
• Variability of components and interactions in plant pathosystem and impact of environmental factors on their expression (spolupracovník)
• Alternative attempts in use of biotechnologies for increased resistance of selected legumes to fungal pathogens (spoluřešitel)
• National program for conservation and utilisation of genetic resources of plants and microorganisms with food and agricultural importance
  B 3.7 Phytopathogenic microorganisms (spoluřešitel)
• Cytological and biochemical aspects of plant-pathogen interactions (model of Lactuca spp.-Bremia lactucae) (řešitel)
• Visual texts for practicals in selected botanical courses (řešitel)
• Cytopathological and pathobiochemical aspects of interactions between plants and fungal pathogens
  (Model interactions: Lactuca spp.-Bremia lactucae and Pisum sativum-Fusarium spp.) (řešitel)

Publications

• Prokopová, J., Špundová, M., Sedlářová, M., Husičková, A., Novotný, R., Doležal, K., Nauš, J. et Lebeda, A. (2010): Photosynthetic responses of lettuce to downy mildew infection and cytokinin treatment. Plant Physiology and Biochemistry, 48, 716-723.
• Ondřej, V., Navrátilová, B., Protivánková, I., Piterková, J., Sedlářová, M., Luhová, L. et Lebeda, A. (2010): Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress. Journal of Experimental Botany, 61, 2395-2401.
• Choi, Y.J., Lebeda, A., Sedlářová, M. et Shin, H.D. (2010): First report of downy mildew caused by Peronospora verbenae on verbena in the Czech Republic. Plant Pathology, 59, 1166.
• Sedlářová, M., Stojaspal, K. et Lebeda, A. (2010): Rozšíření a patogenita Plasmopara halstedii, původce plísně slunečnice, v České republice (Extension and pathogenicity of Plasmopara halstedii, sunflower downy mildew agent, in the Czech Republic). Rostlinolékař 21, 17-20.
• Sedlářová, M., Luhová, L. et Petřivalský, M. (2010): 3rd Plant NO Club. Book of Abstracts, International Meeting, 15.-16.7.2010, Olomouc, Czech Republic; Czech Society for Plant Pathology, Praha and Palacký University in Olomouc, 70 pp.
• Sinha, R.K., Komenda, J., Sedlářová, M. et Pospíšil, P. (2010): Role of PsbH and small chlorophyll-binding proteins in singlet oxygen production in the thylakoid membrane of Synechocystis sp. PCC 6803. Lecture in 6th SPPS PhD Student Conference, Finland, 2.-5.9.2010.
• Sedlářová, M., Lebeda, A., Mikšíková, P., Duchoslav, M., Sedláková, B. et McCreight, J.D. (2009): Histological aspects of Cucumis melo PI 313970 resistance to Podosphaera xanthii and Golovinomyces cichoracearum. - J. Plant. Dis. Protect. 116: 169–176.
• Piterková, J., Petřivalský, M., Luhová, L., Mieslerová, B., Sedlářová, M. et Lebeda, A. (2009): Role of nitric oxide in defence response of Lycopersicon spp. to Oidium neolycopersici infection. Molecular Plant Pathology, 10, 501-513 (Doi: 10.1111/J.1364-3703.2009.00551.X)
• Sedlářová, M., Petřivalský, M., Luhová, L., Piterková, J., Mieslerová, B. et Lebeda, A. (2009): RNOS in pathogenesis of biotrophic fungl plant pathogens. In: Plant ROS 2009 – Plant Oxygen Group meeting on reactive oxygen and nitrogen species, 8.-10.7.2009, Helsinky, Finland, p. 116 (abstract).
• Sedlářová, M., Petřivalský, M., Sedlář, J., Navrátilová, B., Luhová, L. et Lebeda, A. (2009): : Distribution of reactive nitrogen and oxygen species within stressed plant cells. In: Interdisciplinary Symposium on 3 Dimensional Microscopy SSOM, 12.-16.7.2009, Interlaken, Switzerland, p. 144-145 (abstract).
• Mieslerová, B., Sedlářová, M. et Lebeda, A. (2009): Výskyt zástupců čeledi Erysiphaceae (padlí) na okrasných rostlinách na střední Moravě (Occurrence of representatives of family Erysiphaceae (powdery mildews) on ornamental plants in the middle Moravia). In: Šafránková, I., Šefrová, H. (Eds.): XVIII. Česká a slovenská konference o ochraně rostlin. Sborník abstraktů. Mendelova zemědělská a lesnická univerzita v Brně, 2.-4. září 2009, p. 97.
• Sedlářová, M., Petřivalský, M., Luhová, L., Piterková, J., Mieslerová, B. et Lebeda, A. (2009): Reaktivní formy dusíku a kyslíku v patogenezi biotrofních houbových fytopatogenů (Reactive nitrogen and oxygen species in pathogenesis of biotrophic fungal phytopathogens). In: Šafránková, I., Šefrová, H. (Eds.): XVIII. Česká a slovenská konference o ochraně rostlin. Sborník abstraktů. Mendelova zemědělská a lesnická univerzita v Brně, 2.-4. září 2009, p. 120.
• Sedlářová, M., Stojaspal, K., Trojanová, Z., Prokopová, J. et Lebeda, A. (2009): Patogenní variabilita Plasmopara halstedii v ČR a vliv choroby na fotosyntézu náchylných rostlin slunečnice (Pathogenic variability of Plasmopara halstedii in the Czech Republic and influence of the disease on photosynthesis of susceptible sunflower plants). In: Šafránková, I., Šefrová, H. (Eds.): XVIII. Česká a slovenská konference o ochraně rostlin. Sborník abstraktů. Mendelova zemědělská a lesnická univerzita v Brně, 2.-4. září 2009, p. 121.
• Šmehilová, M., Galuszka, P., Bilyeu, K.D., Jaworek, P., Kowalska, M., Šebela, M., Sedlářová, M., English, J.T. et Frébort, I. (2009): Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize. Journal of Experimental Botany 60(9): 2701-2712.
• Sedlář, J., Sedlářová, M. et Flusser, J. (2009): Image Processing Methods for Determination of Downy Mildews from Light Microscopy Images. In: Signal Processing Symposium, 28.-30.5.2009, Warsaw, Poland, 4 pp. (pouze na CD)
• Petřivalský, M., Luhová, L., Piterková, J., Ryzí, M., Sedlářová, M. et Navrátilová, B. (2009): The involvement of nitric oxide and hydrogen peroxide in response of cucumber cells to cadmium. In: Plant ROS 2009 - Plant Oxygen Group meeting on reactive oxygen and nitrogen species, 8.-10.7.2009, Helsinki, Finland, p. 174.
• Sedlářová, M., Stojaspal, K., Trojanová, Z., Prokopová, J. et Lebeda, A. (2009): Plíseň slunečnice (Plasmopara halstedii) v České republice – výskyt, variabilita, vliv na metabolismus hostitelských rostlin (Sunflower downy mildew (Plasmopara halstedii) in the Czech Republic – occurrence, variability and influence on host plant metabolism). Česko-slovenská vědecká mykologická konference, Brno 27.-29.8.2009, Abstrakty. Mykologické listy, Supplementum, p. 55. Česká vědecká společnost pro mykologii, Praha 2009.
• Mieslerová, B., Sedlářová, M. et Lebeda, A. (2009): Výskyt zástupců Erysiphaceae (padlí) na okrasných rostlinách na střední Moravě (Occurrence of Erysiphaceae (powdery mildews) on ornamental plants in the middle Moravia). Česko-slovenská vědecká mykologická konference, Brno 27.-29.8.2009, Abstrakty. Mykologické listy, Supplementum, pp. 84-85. Česká vědecká společnost pro mykologii, Praha 2009.
• Lebeda, A., Mieslerová, B., Sedlářová, M. et Pejchal, M. (2008): Occurrence of anamorphic and teleomorphic stage of Erysiphe palczewskii (syn. Microsphaera palczewskii) on Caragana arborescens in the Czech Republic and Austria and its morphological characterisation. Plant Protect. Sci., 44, 41-48.
• Lebeda, A., Mieslerová, B. et Sedlářová, M. (2008): First report of Erysiphe palczewskii on Caragana arborescens in the Czech Republic. Plant Pathology 57, 779 ((Doi: 10.1111/j.1365-3059.2008.01855.x)
• Lebeda, A., Sedlářová, M., Petřivalský, M. et Prokopová, J. (2008): Diversity of defence mechanisms in plant-oomycete interactions: a case study of Lactuca spp. and Bremia lactucae. Europ. J. Plant Pathol. 122, 71-89. Reprinted in: Lebeda, A., Spencer-Phillips, P.T.N., Cooke, B.M. (Eds.): The Downy Mildews – Genetics, Molecular Biology and Control. Springer, Dordrecht.
• Sedlářová, M., Kitner, M., Lebeda, A., Piterková, J., Luhová, L., Petřivalský, M., Kyseláková, H., Navrátil, M. et Švábová, L. (2008): Reactive nitrogen and oxygen species in pea responses to biotic stress stimuli. In: 2nd International Plant NO Club Workshop, July 21. - 22, (Book of Abstracts). UMR INRA, Université de Bourgogne, Dijon, France, p. 25.
• Petřivalský, M., Kočířová, J., Sedlářová, M., Luhová, L. et Lebeda, A. (2008): The role of NO in the development of Bremia lactucae and lettuce resistance. In: 2nd International Plant NO Club Workshop, July 21. - 22, (Book of Abstracts). UMR INRA, Université de Bourgogne, Dijon, France, p. 36.
• Kyseláková, H., Prokopová, J., Ilík, P., Piterková, J., Luhová, L., Sedlářová, M., Novák, O. et Navrátil, M. (2008): Photosynthetic and oxidative responses of pea leaves to progressive infection due to Pea enation mosaic virus (PEMV). In: International Conference on Biotic Plant Interaction (Book of Abstracts). Queensland Biosciences Precinct, The University of Queensland, Brisbane, Australia, March 27-29, p.84.
• Sedlář, J., Sedlářová, M. et Flusser, J. (2008): Digital Processing of Light Microscopy Images in Plant Pathogen Diagnostics. Mykologické listy 104: p. 53-54 (Abstrakt z výroční konference ČVSM, České Budějovice, 16. února 2008).
• Luhová, L., Vaníčková, P., Piterková, J., Navrátilová, B., Sedlářová, M. et Petřivalský, M. (2008): The role of nitric oxide during regeneration of cucumber cells from protoplast suspension. 14th SFRR 18-22 Oct. 2008, Beijing, China.
• Sedlář, J., Sedlářová, M. et Flusser, J. (2008): Tracking the growth of phytopathogenic fungi and use of digital image processing in diagnostics. In: 9th ICPP, 24-29.8.2008, Torino, Italy (Book of Abstracts). Journal of Plant Pathology 90 (2, Supplement): p. 240.
• Sedlář, J., Sedlářová, M. et Flusser, J. (2008): Digital Processing of Light Microscopy Images in Plant Pathogen Diagnostics. In: Workshop on Bio-Image Informatics: Biological Imaging, Computer Vision and Data Mining (Book of Abstracts). Center for Bio-Image Informatics, UCSB, Santa Barbara, CA, USA, January 17-18, 2008.
• Sedlářová, M., Petřivalský, M., Luhová, M., Hašová, M., Hofman, J., Kočířová, J. et Lebeda, A. (2008): The role of nitric oxide in development of biotrophic pathogens and their interaction with host cells. J. Plant Pathol., 90 (2, Supplement), 2008, S2.240 (Abstract).
• Lebeda, A., Švábová, L., Kitner, M., Digaňová, E., Sedlářová, M., Vinklárková, P. et Griga, M. (2008): Characterization and selection in vitro of peas for resistance to nectrotrophic fungal (Fusarium solani, Ascochyta pisi, Phoma pisi) pathogens – biochemical and morphological approach. In: Prohens, J., Badenes, M.L. (Eds.): Modern Variety Breeding for Present and Future Needs. Editorial Universidad Politécnica de Valencia, Valencia, Spain, pp. 413-414. (Abstract).
• Švábová, L., Klenotičová, H., Griga, M., Lebeda, A., Kitner, M. et Sedlářová, M. (2008): Alternativní biotechnologické postupy ve šlechtění luskovin (Alternative biotechnological approaches in breeding of legumes). Úroda, LVI/12, 59-61.
• Sedlářová, M., Stojaspal, K. et Lebeda, A. (2008): Biologie, rozšíření a patogenita Plasmopara halstedii v České republice (Biology, distribution and pathogenicity of Plasmopara halstedii). In: Nováková, A. (Ed.): Proceedings of the Workshop MICROMYCO 2008. Institute of Soil Biology, Biological Centre, Academy of Sciencev of the Czech Republic, v.v.i., České Budějovice, pp. 82-86.
• Švábová, L., Klenotičová, H., Griga, M., Lebeda, A., Kitner, M. et Sedlářová, M. (2008): Alternativní biotechnologické postupy ve šlechtění luskovin. Úroda LVI(12): 59-61.
• Sedlář, J., Flusser, J. et Sedlářová, M. (2008): Photorealistic Modeling of the Growth of Filamentous Specimens. EURASIP Journal on Advances in Signal Processing, vol. 2008, Article ID 520972, 9 pages, 2008. doi: 10.1155/2008/520972 (dostupne na www http://www.hindawi.com/GetArticle.aspx?doi=10.1155/2008/520972 )
• Lebeda, A., Sedlářová, M., Jankovský, L. et Shin, H.D. (2007): First report of rhododendron powdery mildew on Rhododendron spp. in the Czech Republic. Plant Pathology 56, 354 (Doi: 10.1111/j.1365-3059.2007.01542.x).
• Sedlářová, M., Lebeda, A., Luhová, L. et Grúz, J. (2007): Interactions between Lactuca spp. and Bremia lactucae from histological and biochemical perspective. In: EUCARPIA Leafy Vegetables 2007, Conference Abstracts, 18-20 April 2007, University of Warwick, Warwick HRI, UK; Poster Presentations, p. 23.
• Petřivalský, M., Kočířová, J., Sedlářová, M., Piterková, J., Luhová, L. et Lebeda, A. (2007): On the role of nitric oxide in Bremia lactucae pathogenesis on Lactuca sativa. In: Lebeda, A., Spencer-Phillips, P.T.N. (Eds.): Advances in Downy Mildew Research, Vol. 3. Proceedings of The 2nd International Downy Mildews Symposium. Palacký University in Olomouc and JOLA, v.o.s., Kostelec na Hané (Czech Republic), pp. 175-183.
• Sedlářová, M., Výtisková, M., Doležal, K. et Lebeda, A. (2007): Pre-incubation with cytokinins delays chlorophyll degradation in Lactuca spp. tissues and reduces Bremia lactucae sporulation. In: Lebeda, A., Spencer-Phillips, P.T.N. (Eds.): Advances in Downy Mildew Research, Vol. 3. Proceedings of The 2nd International Downy Mildews Symposium. Palacký University in Olomouc and JOLA, v.o.s., Kostelec na Hané (Czech Republic), pp. 185-192.
• Lebeda, A., Mieslerová, B., Rybka, V., Sedlářová, M. et Petrželová, I. (2007): First record of powdery mildew on Homalocladium platycladum in the Czech Republic. Plant Pathology 56, 722. (Doi: 10.1111/j.1365-3059. 2007.01594.x)
• Lebeda, A., Sedlářová, M. et Mieslerová, B. (2007): Buněčné mechanismy rezistence rostlin vůči patogenům (Cellular mechanisms of plants against pathogens). In: Blahoušek, O. (Ed.): Bulletin České společnosti experimentální biologie rostlin (Bulletin of Czech Society of Experimental Biology of Plants). FGP Studio, Olomouc-Bělidla (Czech Republic), p. 25 (Abstract).
• Prokopová, J., Špundová, M., Sedlářová, M., ., Husičková, A., Doležal, K., Nauš, J. et Lebeda, A. (2007): Vliv cytokininů na změny fotosyntetického aparátu salátu (Lactuca sativa L.) vyvolané plísní salátovou (Bremia lactucae Regel) (Influence of cytokinins on changes of photosynthetic apparatus of lettuce (Lactuca sativa L.) induced by lettuce downy mildew (Bremia lactucae Regel)).In: Blahoušek, O. (Ed.): Bulletin České společnosti experimentální biologie rostlin (Bulletin of Czech Society of Experimental Biology of Plants). FGP Studio, Olomouc-Bělidla (Czech Republic), p. 124 (Abstract).
• Sedlářová, M., Luhová, L., Petřivalský, M. et Lebeda, A. (2007): Localisation and metabolism of reactive oxygen species during Bremia lactucae pathogenesis in Lactuca sativa and wild Lactuca spp. Plant Physiology and Biochemistry, 45, 607-616.
• Prokopová, J., Nauš, J., Špundová, M. et Sedlářová, M. (2007): The effect of meta-topolin on optical parameters of lettuce leaf discs infected by Bremia lactucae. In: Lebeda, A., Spencer-Phillips, P.T.N. (Eds.): Advances in Downy Mildew Research, Vol. 3. Proceedings of The 2nd International Downy Mildews Symposium. Palacký University in Olomouc and JOLA, v.o.s., Kostelec na Hané (Czech Republic), pp. 73-78.
• Grúz, J., Zatloukal, M., Sedlářová, M. et Strnad, M. (2007): Can ultrasound induce systemic resistance in Arabidopsis thaliana? In: 50 Years of the Phytochemical Society of Europe. Churchill College, Cambridge, UK, 11.-14.4.2007, p. 136 (Abstract).
• Vinter, V., Sedlářová, M., Piterková, J., Luhová, L. et Petřivalský, M. (2007): Signální role oxidu dusnatého při klíčení pylu Cucumis spp. In: Blahoušek, O. (Ed.): Bulletin České společnosti experimentální biologie rostlin (Bulletin of Czech Society of Experimental Biology of Plants). FGP Studio, Olomouc-Bělidla (Czech Republic), p. 88 (Abstract).
• Piterková, J., Mieslerová, B., Sedlářová, M., Luhová, L. et Petřivalský, M. (2007): The role of nitric oxide in local and systemic defence response of Lycopersicon spp. to Oidium neolycopersici infection. In: ROS in Plants, Abstract Book. 12.-14.9.2007, Ghent, Belgium, p. 132 (Abstract).
• Novotný, D. et Sedlářová, M. (2007): Fytopatologická mykologie. Mykologické listy 100: 21-23.
• Sedlářová, M., Stojaspal, K. et Lebeda, A. (2007): Biology of Erysiphe azaleae on Rhododendron luteum in the Czech Republic. Mykologické listy 101: 34 (Abstrakt z konference „Houby v antropogenním prostředí“, Praha, 14. dubna 2007)
• Sedlářová, M. et Vinter, V. (2007): Rostlinná pletiva pod tlakem houbových patogenů.- ŽIVA, Praha, (6).
• Lebeda, A., Mieslerová, B., Sedlářová, M., Rybka, V., Petrželová, I. et Doležalová, I. (2007): Nově potvrzené výskyty padlí na okrasných rostlinách v České republice (New records of powdery mildew on ornamental plants in the Czech Republic). In: Nováková, A. (Ed.): Proceedings of the Workshop MICROMYCO 2007. Institute of Soil Biology, Biological Centre, Academy of Sciencev of the Czech Republic, v.v.i., České Budějovice, pp. 74-80. (ISBN 978-80-86525-10-5)
  
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Teaching activities

• Practical in systematics and evolution of lower plants
• General microbiology
• General microbiology
• General and special phytopathology
• Biological seminar
• Microbiology
• Microbiology